Rescaling means that you calculate NRQ values relative to a specified reference level.
Qbase+ allows you to rescale the NRQ values using one of the following as a reference:
After scaling, the expression values of the choice you make here will be set to 1 e.g. when you choose average the average expression level across all samples will be set to 1 and the expression levels of the individual samples will be scaled accordingly.
You can specify the scaling strategy on the Scaling page. Select Scale to group
and set the Scaling group to the untreated
samples . This is one of the reasons why you need the grouping annotation.
Rescaling to the average of a group is typically used to compare results between 2 groups, e.g. treated samples against untreated controls. After rescaling, the average of the NRQs across all untreated samples is 1 and the NRQs of the treated samples are scaled accordingly.
Click Next
to go to the Analysis page.