Research collaborations
Course outline BD
Patent application and Protection
Claims
Patentability requirements
Patent rights
IP Rights
Gene ID mapping
Tools for functional analysis take genes or proteins as input. These genes/proteins are not represented by sequences (unless you are looking for motifs) but by gene or protein IDs. There is an enormous diversity in databases that provide these IDs: Ensembl, Genbank, RefSeq, Uniprot, hugo gene symbols, PDB… Each tool accepts a specific set of […]
Introduction
Licensing
Course outline IP
ONT applications
Slides Human ONT’s white paper on applications of nanopore sequencing in human: Plants ONT’s white paper on applications of nanopore sequencing in plants: Literature
Wet lab workflow
Slides
ONT technology
Slides Technology ONT sequencing is done on flow cells which contain tiny holes (nanopores). Each nanopore corresponds to its own electrode connected to a chip, which measures the electric current that flows through the nanopore. Electrophoresis is used to drive a DNA or RNA strand through the pore. An enzyme controls the rate at which […]
Comparison of platforms
The main characteristics of spatial technologies are: Number of targets and resolution are inversely correlated: Targeted technologies can work with fixed panels of targets or they can allow you to compose your own panel of targets.
Untargeted technologies
Untargeted means that all transcripts or proteins are profiled. They are used to make new discoveries e.g. discover new cell types, new pathways in a tissue…
GDPR
Biobanking
Targeted technologies
Slides Archive (slides from previous editions) Targeted methods profile a specific set of transcripts or proteins. They are typically used to validate hypotheses e.g. confirm spatial differential expression of specific gene
Panels for targeted approaches
Contributors to the content: Clint De Nolf, Cydric Geyskens, Natalia Martinez Viscaino Tools that can help with the design of gene panels: Don’t forget to include the following controls in your panels for cell segmentation, normalization and checking the quality of the data:
