Exercise 4: A Growing Study

@christof Ook hier zitten er foutjes in de markdown. Ik heb die niet aangepast omdat ik deze inhoud toch in een h5p zou steken.

TODO Convert this to h5p

In my qPCR experiment I want to study the pattern of expression of 24 genes (genes of interest and reference genes) in 48 samples (samples of interest and control samples). I want to use 2 PCR replicates for each reaction.

| How many genes can I analyze on one 384 well plate ? |
| :—————————— |
| 48 samples * 2 replicates = 96 reactions per gene.
I can analyze 4 genes on each 384 well plate.

Each week I receive 2 additional samples to analyze.

| Do I analyze them immediately after I get them ? |
| :—————————— |
| No. Since the samples are placed on different plates as in the previous experiment, you have to use IRCs. You typically need 3 IRCs and a no template control sample. It means that if you want to analyze these 2 samples you have to include 4 additional samples for each gene. This is a lot of overhead for just 2 samples !
Try to avoid this: it’s better to wait a few weeks until you have 6 or 8 or even more samples.