The data consist of Cq values for:
Each is measured twice (= technical replicates) in 16 different samples. Half of the samples have undergone a treatment, half of them are untreated control samples.
The data set also contains a series of standard samples consisting of a four-fold dilution series of cDNA for each target gene. These measurements allow to generate a standard curve from which target-specific amplification efficiencies can be calculated. Finally, negative controls (No Template Controls) have been measured. The goal of the analysis is to identify target genes of interest that have different expression levels in the treated samples compared to the untreated control samples.
Import runs button to open the Import Run window
Browse button to go to the directory that stores the files containing the qPCR data
Select the 3 run files simultaneously by holding the
Ctrl key on your keyboard during the selection in Windows or the command button in MacOSX.
Now you go back to the Import Run window, click the
Next button (purple)
In the Imported run names area on the Import run page you should now see the names of the 3 run files. If these are the correct files, click the
Next button at the bottom of the page.